Imobilization of trypsin in films of PVA/CGP
نویسندگان
چکیده
Background Trypsin is an enzyme widely used for digestion of peptides in proteomic research. However, this enzyme is one of the least stable of the neutral proteases and presents rapid autolysis, which causes a decrease in catalytic efficiency and a consequential increase in costs of biotechnological process. Immobilization of the enzyme offers the advantages of enhanced stability, protection against autolysis and the possibility of repeated use of the catalytic material. In addition, the preparation of a successfully immobilized enzyme depends very much on the method of immobilization employed and on the chemical characteristics of the support [1,2]. In this sense, a biodegradable film produced by blending polyvinyl alcohol (PVA) and cashew gum polysaccharides (CGP) was tested as support for trypsin immobilization.
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